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KMID : 1094720150200010051
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Biotechnology and Bioprocess Engineering
2015 Volume.20 No. 1 p.51 ~ p.57
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Marine microbe as nano-factories for copper biomineralization
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Kaur Harneet
Dolma Kunzes
Kaur Navjot
Malhotra Ankit
Kumar Navinder
Dixit Pooja
Sharma Deepak
Mayilraj S.
Choudhury Anirban Roy
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Abstract
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The present research work highlighted the possibility of exploiting marine microbes for the biosynthesis of copper nanoparticles and further investigated thermodynamic changes during the biomineralization of copper in a microbial system by isothermal titration calorimetry. A bacterial strain (M-7) isolated from Kanyakumari coast, India was found capable of synthesizing these nanoparticles. Based on phylogenetic relationship, the strain M-7 was identified as Kocuria flava. The nanoparticles produced were characterized using UV-vis spectrophotometry, Transmission electron microscopy (TEM) and Dynamic light scattering (DLS). Data revealed the formation of spherical and quasi-spherical shaped nanoparticles with an average particle size ranging between 5 and 30 nm. Additionally, a study on the effect of different media components indicated that media containing higher amount of casein enzymic hydrolysate and yeast extract supported the formation of stable colloidal suspension of nanoparticles. Finally, isothermal titration calorimetry (ITC) was carried out to understand the change in heat energy during the formation of nanoparticles in different media. Combinatorial observations of all these studies may open up new strategies to develop tailor made copper nanoparticles via green route.
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KEYWORD
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Kocuria flava, copper nanoparticle, biosynthesis, phylogenetic analysis, isothermal titration calorimetry
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